In vivo detection of H2O2

Confocal microscopy image of a root tip of Arabidopsis thaliana incubated in an hydrogen peroxide indicator, hydroxyphenyl fluorescein (HPF). Excitation (max): 490 nm; Emission (max): 515 nm.

images acquired with the Confocal Microscope Leica SP2;  excitation: 488 nm; emission: 506-586 nm

mz: méristématic zone; ez : elongation zone;
dz: différentiation zone;
Scale : 100 µm

Publication
Dunand C, Crèvecoeur M & C.Penel (2007) – Distribution of superoxide and hydrogene peroxide in Arabidopsis roots and their influence on root development: possible interaction with peroxidases. New Phytol. 174: 332 – 341.