Localization of enzyme activities at the ultrastructural level (Transmission Electron Microscopy)

1/ ATPase activity in shoot apical meristem of Spinacia oleracea

Electron micrograph of cells showing ATPase activity in a few meristematic cells. Most of the ATPase activity is associated to the plasma membrane. It appaers as an electron dense deposit along the membrane. At this magnification the activity associated to other cellular sites is not visible.

2/ Peroxidase activity in leaf cells of Spinacia oleracea.

The activity is associated to the cell wall (1), the plasma membrane (2) and the Golgi (Go).