{"id":400,"date":"2021-12-22T16:28:07","date_gmt":"2021-12-22T15:28:07","guid":{"rendered":"https:\/\/michelecrevecoeur.ch\/?page_id=400"},"modified":"2022-02-02T18:06:11","modified_gmt":"2022-02-02T17:06:11","slug":"polysaccharide-staining-in-paraffin-sections-using-pas-reaction-periodic-acid-schiff","status":"publish","type":"page","link":"https:\/\/michelecrevecoeur.ch\/?page_id=400","title":{"rendered":"Polysaccharide staining using PAS"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"400\" class=\"elementor elementor-400\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6f980a71 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6f980a71\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6c22e90a\" data-id=\"6c22e90a\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6c7bee11 elementor-widget elementor-widget-text-editor\" data-id=\"6c7bee11\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"margin-top: 15px; margin-bottom: 15px; line-height: 25.2px; color: #404040; font-family: arial; font-size: 14px; font-style: normal; font-weight: 400; text-align: center;\"><span style=\"font-size: medium;\"><strong><strong><span style=\"color: #000080;\">Polysaccharide staining of paraffin sections using PAS reaction (<span style=\"text-decoration-line: underline;\">P<\/span>eriodic\u00a0<span style=\"text-decoration-line: underline;\">A<\/span>cid\u00a0<span style=\"text-decoration-line: underline;\">S<\/span>chiff)<\/span><\/strong><span style=\"color: #003366;\"><br \/><\/span><\/strong><\/span><\/p><div style=\"color: #404040; font-family: arial; font-size: 14px;\"><p style=\"margin-top: 15px; margin-bottom: 15px; line-height: 25.2px;\"><span style=\"color: #003366;\">The PAS staining is excellent for localization and identification of carbohydrates on sections. It is specific of different polysaccharides when performed in controlled conditions. The protocol given on this page has been used for staining paraffin sections (8 \u00b5m thick) through plant organs. Two micrographs are shown to illustrate the result of the staining.<\/span><\/p><\/div><p style=\"margin-top: 15px; margin-bottom: 15px; line-height: 25.2px; color: #404040; font-family: arial; font-size: 14px; font-style: normal; font-weight: 400;\"><strong><span style=\"color: #000080;\">T<\/span><\/strong><span style=\"color: #000080;\"><strong>he different steps for Feulgen reaction on paraffin sections are listed here after<\/strong><\/span><\/p><ul style=\"margin-bottom: 10px; color: #404040; font-family: arial; font-size: 14px; font-style: normal; font-weight: 400;\"><li><span style=\"color: #003366;\">Deparaffinize and rehydrate to water<\/span><\/li><li><span style=\"color: #003366;\">Oxidize in 0,5 % periodic acid solution for 15 min at room temperature. A control section without treatment is immersed in water.<\/span><\/li><li><span style=\"color: #003366;\">Wash in tap water for 10 min then in distilled water<\/span><\/li><li><span style=\"color: #003366;\">Stain with Schiff\u2019s reagent at darkness and free of oxygen. The time of staining varies according to the tissue and the fixative. For example: 15 min for sections through\u00a0<em>Zea mays<\/em>\u00a0embryos fixed with FAA and using a fresh Schiff\u2019s reagent.<\/span><\/li><li><span style=\"color: #003366;\">Sulfurous water to remove excess of unfixed Schiff\u2019s reagent.<\/span><\/li><li><span style=\"color: #003366;\">Rince in distilled water.<\/span><\/li><li><span style=\"color: #003366;\">Dehydrate, clear in xylene and mount in Eukitt or other appropriate mounting medium.<strong>\u00a0<\/strong><\/span><\/li><li><span style=\"color: #003366;\">Observe under the microscope. The carbohydrates are stained dark pink<\/span><\/li><\/ul><p style=\"margin-top: 15px; margin-bottom: 15px; line-height: 25.2px; color: #404040; font-family: arial; font-size: 14px; font-style: normal; font-weight: 400;\"><span style=\"color: #003366;\"><strong>Principle of the staining<\/strong><\/span><\/p><p style=\"margin-top: 15px; margin-bottom: 15px; line-height: 25.2px; color: #404040; font-family: arial; font-size: 14px; font-style: normal; font-weight: 400;\"><span style=\"color: #003366;\">The oxidative process results in the formation of aldehyde groups through carbon-to-carbon bond cleavage from neighbor\u2019s hydroxyl groups. Aldehydes are revealed by the pink coloration as result of their reaction with the colorless Schiff\u2019s reagent (a mixture of pararosaniline and sodium metabisulfite). PAS is an abbreviation for\u00a0<\/span><strong style=\"color: #003366;\">P<\/strong><span style=\"color: #003366;\">eriodic &#8211;\u00a0<\/span><strong style=\"color: #003366;\">A<\/strong><span style=\"color: #003366;\">cid \u2013\u00a0<\/span><strong style=\"color: #003366;\">S<\/strong><span style=\"color: #003366;\">chiff. Periodic acid that remains in the section after oxidation may be reduced by a treatment with sodium thiosulfate \u2013 potassium iodate. In this case the staining is designated as PARS for\u00a0<\/span><strong style=\"color: #003366;\">P<\/strong><span style=\"color: #003366;\">eriodic\u00a0<\/span><strong style=\"color: #003366;\">A<\/strong><span style=\"color: #003366;\">cid\u00a0<\/span><strong style=\"color: #003366;\">R<\/strong><span style=\"color: #003366;\">educing\u00a0<\/span><strong style=\"color: #003366;\">S<\/strong><span style=\"color: #003366;\">chiff.<br \/><\/span><span style=\"color: #003366;\">Carbohydrates that are stained are polymers of insoluble glucose such as glycogen, starch, and cellulose.<\/span><\/p><p style=\"margin-top: 15px; margin-bottom: 15px; line-height: 25.2px; color: #404040; font-family: arial; font-size: 14px; font-style: normal; font-weight: 400;\" align=\"center\"><span style=\"color: #003366;\"><strong>Below : Part of a cross section through a\u00a0<em>Zea mays<\/em>\u00a0embryo<\/strong><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e03e5bd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e03e5bd\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1e9e6b3\" data-id=\"1e9e6b3\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b85f7db elementor-widget elementor-widget-image\" data-id=\"b85f7db\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"549\" height=\"408\" src=\"https:\/\/michelecrevecoeur.ch\/wp-content\/uploads\/2021\/12\/endosperme-2.jpg\" class=\"attachment-large size-large wp-image-340\" alt=\"\" srcset=\"https:\/\/michelecrevecoeur.ch\/wp-content\/uploads\/2021\/12\/endosperme-2.jpg 549w, https:\/\/michelecrevecoeur.ch\/wp-content\/uploads\/2021\/12\/endosperme-2-300x223.jpg 300w\" sizes=\"(max-width: 549px) 100vw, 549px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Starch grains (a: arrow) and cell walls are stained pink. Nuclei (n) and cytoplasm are not stained.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-fdea6b2 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fdea6b2\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-a42354e\" data-id=\"a42354e\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1ae766d elementor-widget elementor-widget-image\" data-id=\"1ae766d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"488\" height=\"480\" src=\"https:\/\/michelecrevecoeur.ch\/wp-content\/uploads\/2021\/12\/PAS-staining-2.jpg\" class=\"attachment-large size-large wp-image-402\" alt=\"\" srcset=\"https:\/\/michelecrevecoeur.ch\/wp-content\/uploads\/2021\/12\/PAS-staining-2.jpg 488w, https:\/\/michelecrevecoeur.ch\/wp-content\/uploads\/2021\/12\/PAS-staining-2-300x295.jpg 300w\" sizes=\"(max-width: 488px) 100vw, 488px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\"><\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-0cb922d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"0cb922d\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-3b5018a\" data-id=\"3b5018a\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-abdfe83 elementor-widget elementor-widget-text-editor\" data-id=\"abdfe83\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #404040; font-family: arial; font-size: 14px;\">The cell walls of the different tissues are all pink stained. The\u00a0<\/span><a href=\"https:\/\/michelecrevecoeur.ch\/?page_id=296\">collenchyma<\/a><span style=\"color: #404040; font-family: arial; font-size: 14px;\">\u00a0staining is more intense as result of the cellulosic thickening of the walls of this support tissue. (col: angular collenchyma). Blue arrow: epidermis with its cuticle (pink arrow).<\/span><strong style=\"color: #404040; font-family: arial; font-size: 14px;\">\u00a0 \u00a0\u00a0<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Polysaccharide staining of paraffin sections using PAS reaction (Periodic\u00a0Acid\u00a0Schiff) The PAS staining is excellent for localization and identification of carbohydrates on sections. It is specific of different polysaccharides when performed in controlled conditions. The protocol given on this page has been used for staining paraffin sections (8 \u00b5m thick) through plant organs. Two micrographs are [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-400","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Polysaccharide staining using PAS - Mich\u00e8le Cr\u00e8vecoeur<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/michelecrevecoeur.ch\/?page_id=400\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Polysaccharide staining using PAS - Mich\u00e8le Cr\u00e8vecoeur\" \/>\n<meta property=\"og:description\" content=\"Polysaccharide staining of paraffin sections using PAS reaction (Periodic\u00a0Acid\u00a0Schiff) The PAS staining is excellent for localization and identification of carbohydrates on sections. 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It is specific of different polysaccharides when performed in controlled conditions. The protocol given on this page has been used for staining paraffin sections (8 \u00b5m thick) through plant organs. 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