{"id":1670,"date":"2022-01-26T13:36:17","date_gmt":"2022-01-26T12:36:17","guid":{"rendered":"https:\/\/michelecrevecoeur.ch\/?page_id=1670"},"modified":"2022-02-14T18:14:39","modified_gmt":"2022-02-14T17:14:39","slug":"atomic-force-microscopy","status":"publish","type":"page","link":"https:\/\/michelecrevecoeur.ch\/?page_id=1670","title":{"rendered":"Atomic Force Microscopy"},"content":{"rendered":"\t\t
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Atomic Force Microscopy.<\/strong><\/span><\/p>

The Atomic Force Microscope designated by the abbreviation \u00ab\u00a0AFM\u00a0\u00bb belongs to the family of near field microscopes or local probes microscopes. It is used to obtain high resolution images, on the nanometer scale, of the surface of biological samples. It allows to observe and image in air samples but also in liquid, physiological conditions. In addition, samples viewed by AFM do not need to be conductors and do not require any preparation that would change or damage the sample.
The resolution of AFM is high: lateral 0,5 -1 nm lateral; vertical 0,01 nm lateral for dry samples and 0,1 \u2013 0,2 nm for samples imaged in physiological conditions. AFM can not only give image in the 3 D topography, but it also provides qualitative and quantitative information and different physical properties (surface texture and roughness; size).<\/p>

A sharp tip attached to a cantilever is scanned over the sample surface and moves in response to tip \u2013 surface interactions at the atom level, the movement being measured with a laser beam photodiode. Different forces are involved in the imaging process.<\/p>

Below a few AFM pictures of (1)\u00a0 purified membranes from spinach leaves observed in buffer and (2) polygalacturonic acid molecules observed in air after drying (protocol for preparation<\/a> in French).<\/p>

See the pdf Document in French \u00a0\u00bb\u00a0AFM en biologie<\/a>\u00a0\u00bb\u00a0– cours de Microscopie \u00e9lectronique –\u00a0 M. CREVECOEUR UniGe<\/p>

Membranes from spinach leaf cells imaged by AFM
<\/strong>See the publication \u00ab\u00a0Cr\u00e8vecoeur M et al. (2000) – Protoplasma 212: 46-55\u00a0[pdf<\/a>]\u00a0\u00bb<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Spinach plasma membranes have been imaged with a Nanoscope III microscope - laboratory of Prof. Eric Lesniewska - University of Burgoundi - Dijon - France<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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 Polygalacturonic acid mol\u00e9cules observed dry on mica<\/strong><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Atomic Force Microscopy. The Atomic Force Microscope designated by the abbreviation \u00ab\u00a0AFM\u00a0\u00bb belongs to the family of near field microscopes or local probes microscopes. It is used to obtain high resolution images, on the nanometer scale, of the surface of biological samples. It allows to observe and image in air samples but also in liquid, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-1670","page","type-page","status-publish","hentry"],"yoast_head":"\nAtomic Force Microscopy - Mich\u00e8le Cr\u00e8vecoeur<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/michelecrevecoeur.ch\/?page_id=1670\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Atomic Force Microscopy - Mich\u00e8le Cr\u00e8vecoeur\" \/>\n<meta property=\"og:description\" content=\"Atomic Force Microscopy. 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