{"id":125,"date":"2021-12-05T15:16:59","date_gmt":"2021-12-05T14:16:59","guid":{"rendered":"https:\/\/michelecrevecoeur.ch\/?page_id=125"},"modified":"2022-02-26T17:39:03","modified_gmt":"2022-02-26T16:39:03","slug":"expertis","status":"publish","type":"page","link":"https:\/\/michelecrevecoeur.ch\/?page_id=125","title":{"rendered":"Expertise"},"content":{"rendered":"\t\t
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Areas of expertise: microscopy; histo\/cytology; histo\/cytochemistry; plant anatomy<\/strong><\/span><\/p>

The years spent at university allowed me to specialize and gain experiences of a wide variety of techniques in the field of microscopy applied to study of plants.
Techniques listed below have been partly developed to respond to the needs of\u00a0various projects in which I was involved<\/strong>\u00a0in laboratories of the Department of Botany and Plant Biology (Geneva University) but also for collaborations with other laboratories in Switzerland or in France.
Among these techniques I will cite the localization of gene expression by in situ hybridization on paraffin section using a non-radioactive method (DIG) and the comparison at the tissue level (light microscopy) and cellular level (light and electron microscopy) of plant phenotypes (see\u00a0Research<\/strong><\/a>).
The different techniques were also made available to master and PhD students participating in my curses and practical curses : \u201cHistochemistry and Cytochemistry\u201d and \u201c Introduction to Electron Microscopy\u201d (see Teaching).<\/p>

Light Microscopy<\/strong><\/span><\/p>

In this field, various techniques for preparing plant samples have been developed and were used routinely in view of their observation and of their histological and cytological study. They involve sectioning very different plant samples: flowers, siliques, stems, roots, seeds, root nodule. The sections were made in (1) fresh material with either a razor blade<\/a> or a hand microtome<\/a> or with a vibratome;<\/a> (2) frozen samples, with a cryostat; (3) samples fixed and included un different media: paraffin, technovit 7100, epoxy resins (Epon, Spurr) and acrylic resins (LR White) using a rotary microtome<\/a>.
The thickness of the sections varies according to the technique, from 100 \u00b5m (freehand and vibratome sections) to 1 \u00b5m (sections in resins). The fixation and inclusion methods vary, of course, depending on the objective.
According to the type of sections, protocols for staining and for cell study have been developed and were used routinely. They are listed here.<\/p>