{"id":117,"date":"2021-12-05T14:54:04","date_gmt":"2021-12-05T13:54:04","guid":{"rendered":"https:\/\/michelecrevecoeur.ch\/?page_id=117"},"modified":"2022-02-26T17:10:51","modified_gmt":"2022-02-26T16:10:51","slug":"research","status":"publish","type":"page","link":"https:\/\/michelecrevecoeur.ch\/?page_id=117","title":{"rendered":"Research"},"content":{"rendered":"\t\t
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Research Activities at Geneva University from 2004 to 2013<\/strong><\/h5>

From 2004 to 2013 I collaborated with different research groups and for each of these collaborations I took charge of the histo\/cytological experiments, from the sample selection until the micrographs and their interpretation. All the micrographs on this site related to these collaborations concern wild phenotypes.<\/span><\/span><\/p>

Collaborations with research groups at the Department of Botany and Plant Biology\u00a0
<\/strong><\/span><\/h5>

1.<\/strong>\u00a0Groups of professors Jean David Rochaix and Michel Goldschmidt<\/span> – Clermont\u00a0\u2013\u00a0Laboratory of Molecular Genetic of Chloroplasts\u00a0\u2013 From 2004 to 2013.<\/span><\/p>

Study of membrane assembly of chloroplasts in\u00a0the unicellular green alga Chlamydomonas<\/em> reinhardtii<\/em><\/a> \u00a0and\u00a0the flowering land plant Arabidopsis thaliana<\/em><\/a>, in wild type and mutants phenotypes affected in the formation of thylakoids. Cellular approach using transmission electron microscopy, immunofluorescence and confocal microscopy.<\/span><\/p>

Collaborations with:<\/strong>\u00a0Dr Freddy Barn\u00e8che; PhD Students: Vera G\u00f6hre, Iga Samol; Silvia Ramundo.<\/span>
Publications:<\/strong>\u00a0Barn\u00e8che F et al. (2006)\u00a0EMBO J; G\u00f6hre V et al. (2006)\u00a0Plant Cell; Fristedt R et al. (2009)\u00a0Plant Cell.;\u00a0Samol I.et al. (2012). Plant Cell ;\u00a0Ramundo S et al. (2014). Plant cell.<\/span><\/p>

2.<\/strong>\u00a0Group of Professor Teresa Fitzpatrick\u00a0\u2013\u00a0Plant Biochemistry and Physiology Laboratory. Metabolism of vitamins in plants – From 2010 to 2013. Study of B6 vitamin (effect on the growth of the embryo<\/a>) and of vitamin B1 (transport)\u00a0in Arabidopsis, wild types and mutants.\u00a0Cellular approach: transmission electron microscopy, light and confocal microscopy.<\/span>
Collaborations with:<\/strong>\u00a0Svetlana Boycheva (PhD student); Dr Elisabeth Gas -Pascual.<\/span>
Publication:<\/strong>\u00a0Raschke M et al. (2011)\u00a0Plant J.; Martinis J et al. (2016)<\/span><\/p>

3.<\/strong>\u00a0Group of Dr\u00a0Xavier Perret<\/span>\u00a0– Laboratory of Microbial Genetics – From 2009 to 2012. Cytological study of nodules induced on roots of different legume plants (Lotus japonicus,\u00a0Vigna u\u00a0;\u00a0Leucaena leucocephala<\/a>\u00a0;\u00a0Tephrosia vogelii)<\/em>\u00a0by the bacteria\u00a0Rhizobium\u00a0sp. NGR234 (a bacteria with a broad spectrum of host plants) and different mutants of this bacteria strain, using light and transmission electron microscopy.<\/span>
Collaborations\u00a0with:<\/strong>\u00a0Nadia Bakkou (PhD Student); Dr M. Saad.<\/span>
Publication:<\/strong>\u00a0Saad M.et al.(2012).\u00a0Appl Environ Microbiol.; Saad M et al. (2019) Molecular Plant-Microbe Interactions.<\/span><\/p>

4.<\/strong>\u00a0Group of Professor Jurek Paszkowski\u00a0<\/span>\u2013 Laboratory of Plant Genetic \u2013 From 2008 to 2012. Determination and localization of a retro transposon ONSEN activated by heat stress in Arabidopsis thaliana.<\/em> Development of anthers<\/a> in Arabidopsis th.<\/em>, wild type and mutant.<\/span>
Approach:<\/strong>\u00a0light microscopy\u00a0; transmission electron microscopy\u00a0; epi fluorescence.<\/span>
Collaboration with:<\/strong>\u00a0Drs Etienne Bucher, Marco Catoni; Herv\u00e9 Gauber (PhD student).<\/span><\/p>

5.<\/strong>\u00a0 Group of Professor Luis Lopez -Molina<\/span> \u2013 Seed Molecular Genetics \u2013 From 2009 to 2011.\u00a0Control of germination <\/a>of Arabidopsis thaliana<\/em> seeds. R\u00f4le of abscissic acid in Arabidopsis thaliana<\/em>, seeds from wild type and abi5 mutants.<\/span>
Approach:<\/strong>\u00a0light microscopy; transmission electron microscopy<\/span>
Collaborations:<\/strong>\u00a0Drs Natsuko; U. Piskurewicz<\/span><\/p>

6.<\/strong>\u00a0Group of Professor\u00a0Claude Penel<\/span>\u00a0\u2013\u00a0Laboratory of Plant Physiology – From 2004 to 2006. In situ<\/em>\u00a0detection of Reactive oxygen species in Arabidopsis<\/em> roots using HPF<\/a> (hydroxyphenyl\u00a0fluorescein) as fluorescent detector. Observations using confocal microscopy.\u00a0<\/span>
Publication:<\/strong>\u00a0Dunand C. et al. (2007)\u00a0New Phytol.<\/span><\/p>

7.<\/strong>\u00a0Group of Professor William Broughton<\/span>\u00a0\u2013 Molecular Biology of Superior Plants \u2013 From 2006 to 2009. Cytological (light and transmission electron microscopy) study of nodules induced on roots of Lotus japonicus<\/em>\u00a0and\u00a0Vigna unguiculata:<\/em> cellular comparative study of nodules induced by (1) Mezorhizobium loti<\/em> and Rhizobium sp.<\/em> NGR234\u00a0on host plant\u00a0Lotus japonicus<\/em>\u00a0; (2) by Rhizobium<\/em> sp.NGR234 (wild type and mutants strains of surface polysaccharides) on the host plant Vigna unguiculata<\/em>.<\/span>
Collaborations with:<\/strong>\u00a0Drs Olivier Schumpp, Antoine Le Qu\u00e9r\u00e9 and William Deakin.<\/span>
Publication:<\/strong>\u00a0Schumpp O et al. (2008)\u00a0J Exp Bot.<\/span><\/p>

Collaboration with\u00a0Dr Nicolas Capelli\u00a0
<\/a>Laboratory of Chrono-Environment\u00a0(UMR CNRS 6249 usc INRA)<\/span> University of \u00ab\u00a0Franche – Comt\u00e9\u00a0\u00bb (Fr).<\/span><\/h6>

From 2007 \u2013 2012. \u00abStudy of the cellular response of different forest species to flooding stress\u00a0\u00bb. Problematic: in France it is estimated that 2 million hectares of soil are exposed to flooding. This mainly induces an oxygen deficit in the soil (hypoxy or anoxy) which is harmful for the roots. Little is still known about the nature of response of woody trees to this kind of stress. Pedunculate oak (Quercus robur\u00a0L) and sessile oak (Quercus petrae\u00a0L.), two most common woody species in central Europa, show an opposite tolerance against ou towards flooding. The objective was the study of cellular and molecular bases of the difference of sensibility of these two species towards different periods of flooding. Initially this collaboration focused on the study of non – symbiotic hemoglobin expression and on cellular modifications in roots of the two oak species submitted to short and long flooding periods. It was performed by a Master Student Audrey BERGER (University of Geneva, 2007). The title of this work was \u201cIn situ<\/em> hybridization study of no symbiotic hemoglobin in two oak ecotypes in response to flooding\u201d.<\/span><\/p>

Publications:<\/strong>\u00a0Parent C et al. (2008)\u00a0New Phytol\u00a0; Parent et al. (2008)\u00a0Plant Signaling & Behavior;\u00a0Parent et al. (2011)\u00a0Plant Cell Environment.<\/span>
This collaboration then focused on the study of genes involved in mechanisms of regulation of water flow among which aquaporins but also in programmed cellular death. The study was multidisciplinary\u00a0using both molecular and cellular biology. See\u00a0the thesis of Claire Depardieu in Besan\u00e7on<\/a><\/strong>\u00a0.<\/span><\/span>
Publication:<\/strong>\u00a0C. Rasheed-Depardieu et al.\u00a0PLoS One\u00a0(2012) open access on line.<\/span><\/p>

Collaboration with the professor Herve Vanderschuren<\/span><\/p>

Department of Biology – Institute of Agricultural Sciences – Group of Plant Biotechnology- Cassava Research – ETH Zurich – From 2010 to 2011. Histology of Cassava roots<\/span>.<\/p>

In progress<\/strong>\u00a0: my research activities from 1975 to 2003.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Research Activities at Geneva University from 2004 to 2013 From 2004 to 2013 I collaborated with different research groups and for each of these collaborations I took charge of the histo\/cytological experiments, from the sample selection until the micrographs and their interpretation. All the micrographs on this site related to these collaborations concern wild phenotypes. […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-117","page","type-page","status-publish","hentry"],"yoast_head":"\nResearch - 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